Qγ−H2AX, an analysis method for partial-body radiation exposure using γ-H2AX in non-human primate lymphocytes
نویسندگان
چکیده
منابع مشابه
In vivo Analysis of H2AX Phosphorylation Induced by γ-Radiation
ABSTRACT Background and Objectives: Exposure to ionizing radiation in modern societies is inevitable and can cause a variety of adverse health effects such as cancer and birth defects. Therefore, a reliable, repeatable and sensitive method is required for evaluation of radiation exposure. The aim of this study was to determine the amount of hist...
متن کاملGamma-H2AX-Based Dose Estimation for Whole and Partial Body Radiation Exposure
Most human exposures to ionising radiation are partial body exposures. However, to date only limited tools are available for rapid and accurate estimation of the dose distribution and the extent of the body spared from the exposure. These parameters are of great importance for emergency triage and clinical management of exposed individuals. Here, measurements of γ-H2AX immunofluorescence by mic...
متن کاملγ-H2AX as a protein biomarker for radiation exposure response in ductal carcinoma breast tumors: Experimental evidence and literature review
Background: H2AX is a histone variant that is systematically found and ubiquitously distributed throughout the genome. DNA double-strand breaks (DSBs) induce phosphorylation of H2AX at serine 139 (γH2AX), an immunocytochemical assay with antibodies recognizing γH2AX has become the gold standard for the detection of DSBs. The importance of this assay to investigate different individu...
متن کاملThe Use of Gamma-H2AX as a Biodosimeter for Total-Body Radiation Exposure in Non-Human Primates
BACKGROUND There is a crucial shortage of methods capable of determining the extent of accidental exposures of human beings to ionizing radiation. However, knowledge of individual exposures is essential for early triage during radiological incidents to provide optimum possible life-sparing medical procedures to each person. METHODS AND FINDINGS We evaluated immunocytofluorescence-based quanti...
متن کاملCalibration of the γ-H2AX DNA Double Strand Break Focus Assay for Internal Radiation Exposure of Blood Lymphocytes
DNA double strand break (DSB) formation induced by ionizing radiation exposure is indicated by the DSB biomarkers γ-H2AX and 53BP1. Knowledge about DSB foci formation in-vitro after internal irradiation of whole blood samples with radionuclides in solution will help us to gain detailed insights about dose-response relationships in patients after molecular radiotherapy (MRT). Therefore, we studi...
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ژورنال
عنوان ژورنال: Radiation Measurements
سال: 2011
ISSN: 1350-4487
DOI: 10.1016/j.radmeas.2011.02.017